News

imagesCA718DSO

Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials

The conventional dye and fluorescence-based cytotoxicity assays are limited by the interference of such readouts with nanomaterials

Nanotoxicology. February 2014, Vol. 8, No. 1 , Pages 50-60

Andrea Neumeyer1,4, Mirko Bukowski2, Michael Veith2, Claus-Michael Lehr1,3, Nicole Daum 1,3  

1 Biopharmaceutics and Pharmaceutical Technology, Saarland; niversity, Saarbrücken, Germany 2 INM – Leibniz Institute for New Materials, Saarbrücken, Germany; 3 Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Center for Infection Research, Saarbrücken, Germany; 4 Institute for Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.

Abstract

Investigating the safety of nanoparticles is essential for many fields of their applications, in particular for consumer products, food and medicines. The conventional dye and fluorescence-based cytotoxicity assays are limited by the interference of such readouts with nanomaterials. This holds in particular when nanomaterials have been fluorescently labelled for other purposes, for example, confocal microscopy. Moreover, most of these assays are invasive, that is, typically involve irreversible changes or destruction of cells and hence only allowing one endpoint measurement. Therefore, a non-invasive method for the detection of cytotoxicity was developed which is based on the automated online monitoring of the oxygen concentration in solution (SensorDish® Reader). Fluorescently labelled silica nanoparticles with different sizes and surface modifications were used as model systems to explore this novel assay. Thereby, the SensorDish® Reader allows a life documentation of the cellular behaviour and clarifies that size, time, concentration and surface modification of nanoparticles affect cellular viability.